Journal: Frontiers in Surgery

Article Title: Comparative analysis of adolescent intramedullary nailing and locking plate fixation for femoral shaft fractures

doi: 10.3389/fsurg.2025.1614146

Figure Lengend Snippet: Comparisons of serum IL-6 (A) , IL-1β (B) , IL-8 (C) , PGE2 (D) and pain material P (E) between the two groups at the time of 1 week post-operation. n = 62 for each group. Data were shown with mean ± SD. ** p < 0.01, *** p < 0.001 from Unpaired t test with Welch's correction.

Article Snippet: The following kits were used: Human IL-6 ELISA Kit (Cat# EH0201), Human IL-1β ELISA Kit (Cat# EH0185), Human IL-8 ELISA Kit (Cat# EH0205), Human PGE2 ELISA Kit (Cat# EH4233)—all purchased from Wuhan Fine Biotechnology Co., Ltd (Wuhan, China).

Techniques:

Journal: Cancer Research

Article Title: Ferroptotic Neutrophils Induce Immunosuppression and Chemoresistance in Breast Cancer

doi: 10.1158/0008-5472.CAN-24-1941

Figure Lengend Snippet: Cross-talk between neutrophils and Fer-CD4 T cells maintained extensive neutrophil ferroptosis. A, Gene Ontology (GO) terms associated with upregulated genes of C2_IL1B cluster in scRNA-seq. B, Gene set enrichment analysis of bulk RNA-seq revealed enrichment of neutrophil chemotaxis genes in chemoresistant CD4 TILs. C, Scheme of chemotaxis assays with Boyden transwell chambers. D, Representative images (left) and quantification (right) of chemotaxis assays for peripheral neutrophils toward CM of different CD4 TILs. Scale bar, 100 μm. E, Migration tracks of neutrophils in μ-slide chemotaxis experiments toward CM of non–Fer-CD4 or Fer-CD4 T cells. F, Representative fluorescent images of cytoskeleton staining (left) and quantification (right) of filopodium-like protrusions (FLP) of peripheral neutrophils in the presence of CM from non–Fer-CD4 or Fer-CD4 T cells. Scale bar, 5 μm. G, Ex vivo tumor slice migration assays for the recruitment of CFSE-labeled neutrophils into breast tumor slices with high or low density of Fer-CD4 T cells. Scale bar, 100 μm. H, Dot plot for the expression of various chemokines in C2_IL1B cells in scRNA-seq. I, ELISA for CXCL3, IL8, and S100A9 release of non–Fer-CD4 or Fer-CD4 T cells. J and K, Migration ( J ) and filopodium-like protrusions ( K ) of peripheral neutrophils in the presence of CM from Fer-CD4 T cells and different antibodies. L, Cell death of peripheral neutrophils in the presence of CM of Fer-CD4 T cells and different antibodies categorized by location (top or bottom chamber of transwell system). M, CXCL3 and IL1β protein level of naïve CD4 T cells by immunoblotting after coculturing with control or ferroptotic neutrophils, which were generated by engagement with non–Fer-CD4 or Fer-CD4 T cells, respectively. Results are represented as mean ± SD of n = 7 ( D ) or n = 5 ( F and I–M ) or n = 6 ( G ). *, P < 0.05; **, P < 0.01; ***, P < 0.001 by two-sided one-way ANOVA with the Tukey test ( D , F , and J–L ) or Student t test ( G , I , and M ). Representative images are shown in Supplementary Fig. S7 ( J and K ).

Article Snippet: Human IL-1β ELISA Kit (Invitrogen, #BMS224-2), Human CXCL3 ELISA Kit (Biorbyt, #orb405701), Human IL-8 ELISA Kit (R&D Systems, #D8000C), Human S100A9 ELISA kit (R&D Systems, #DY5578), Human PGE2 ELISA kit (Invitrogen, #KHL1701), Human IDO ELISA Kit (R&D Systems, #DY6030B), Human HGF ELISA Kit (R&D Systems, #DHG00B), Human TGF-beta1 ELISA Kit (R&D Systems, #DB100C), Human IL-10 ELISA Kit (R&D Systems, #D1000B), Human IL-21 ELISA Kit (R&D Systems, #DY8879), Human EBI3 ELISA Kit (R&D Systems, #DY6456), Human IL-6 ELISA Kit (R&D Systems, #D6050B), Human IL-23 ELISA Kit (R&D Systems, #D2300B), and Human IL-17 ELISA Kit (R&D Systems, #QK317) were utilized following the manufacturer’s instructions.

Techniques: RNA Sequencing Assay, Chemotaxis Assay, Migration, Staining, Ex Vivo, Labeling, Expressing, Enzyme-linked Immunosorbent Assay, Western Blot, Control, Generated